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Comparison of <t>BDNF,</t> NSE, and FABP5 levels in brain tissues of rats across groups. (A) Western blot analysis of BDNF, NSE, and FABP5 protein expression in the brain tissues of each group. (B) Immunofluorescence detection of FABP5 in brain tissues of each group. Green fluorescence indicates FABP5 protein expression. (C–E) Western blot and relative quantification of NSE, FABP5, and BDNF expression levels in each group. (F–H) Statistical analysis of mRNA levels of NSE, FABP5, and BDNF in each group.
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Comparison of BDNF, NSE, and FABP5 levels in brain tissues of rats across groups. (A) Western blot analysis of BDNF, NSE, and FABP5 protein expression in the brain tissues of each group. (B) Immunofluorescence detection of FABP5 in brain tissues of each group. Green fluorescence indicates FABP5 protein expression. (C–E) Western blot and relative quantification of NSE, FABP5, and BDNF expression levels in each group. (F–H) Statistical analysis of mRNA levels of NSE, FABP5, and BDNF in each group.

Journal: Open Life Sciences

Article Title: Resveratrol modulates FABP5 to reduce neuronal apoptosis following ischemic stroke

doi: 10.1515/biol-2025-1253

Figure Lengend Snippet: Comparison of BDNF, NSE, and FABP5 levels in brain tissues of rats across groups. (A) Western blot analysis of BDNF, NSE, and FABP5 protein expression in the brain tissues of each group. (B) Immunofluorescence detection of FABP5 in brain tissues of each group. Green fluorescence indicates FABP5 protein expression. (C–E) Western blot and relative quantification of NSE, FABP5, and BDNF expression levels in each group. (F–H) Statistical analysis of mRNA levels of NSE, FABP5, and BDNF in each group.

Article Snippet: Further, radioimmunoprecipitation assay lysis buffer (ES-8150; Ecotop, China), bicinchoninic acid protein assay kit (EK-5001; Ecotop), polyvinylidene difluoride membrane (ISEQ00010; Millipore, USA), AMPKa1/AMPKa2 mouse mAb (1:1000, A27099 , ABclonal, China), BDNF polyclonal (1:1000, 28205-1-AP; Proteintech, China), FABP4 (1:2000, ab92501; Abcam, England), FABP5 (1:1000, ab151772; Abcam, and FABP3) (1:800, A8789; ABclonal) were used in the study.

Techniques: Comparison, Western Blot, Expressing, Immunofluorescence, Fluorescence, Quantitative Proteomics